臭氧氧化預處理對大鼠肝缺血再灌注損傷的保護作用

李糧輝  陳文華  李麗珍  陳燕凌  王蘭蘭

【摘要】目的 評價臭氧氧化預處理對大鼠肝臟缺血再灌注損傷的影響。方法 將18只SD 大鼠（雄性，8周齡，250 -300 g）按隨機數字表法分為3組：假手術組（S組）、缺血再灌注組（I/R組） 和臭氧預處理組（O3+I/R組），每組6只。O3+I/R組于術前5天連續腹腔注射臭氧和氧氣混合氣體（臭氧濃度為50 mg/L,1 mg?kg -1?d-1 ）。采用結扎肝動脈、門靜脈和膽管分支45 min后恢復灌注的方法制備大鼠肝臟缺血再灌注損傷模型。再灌注3 h后抽取腹主動脈血樣測定血清ALT和AST 水平；處死大鼠取肝組織，測定超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。結果 與S組比較，I/R組和O3+I/R組血清ALT和AST水平、肝組織MDA含量升高，I/R組肝組織SOD活性降低 （P<0.05），O3 +I/R組肝組織SOD活性升高（P<0.05）；與I/R組比較，O3+I/R組血清ALT和AST 水平、肝組織MDA含量降低，肝組織SOD活性升高。結論 臭氧預處理可減輕大鼠肝臟缺血再灌注損傷，其機制可能與抑制脂質過氧化反應有關。

　　【關鍵詞】臭氧氧化預處理；缺血再灌注；肝臟

　　基金項目：福建省自然科學基金（2016J01543）

　　Ozone oxidative intervention protects against hepatic ischemia reperfusion injury in rats

　　Li Liang- hui* , Chen Wenhua, Li Lizhen, Chen Yanting, Wang Lanlan.

　　* Department of Anesthesiology, Southern Branch O f the Fujian Proincial Hospital, Fuzhoo 350028 , China

　　Correspooding author: Chen Wenhua, DepaOment O f Anesthesiology, Fujian Medical Universita Unioo Hospital, Emaii: whc6202@ 163. com

　　【Abstract】 Objective To explore the protective effect of ozone oxidative preconditioning on hepatic ischemia reperfusion injury in rats. Methodcs Eighteen 8-week-old SPF Sprague-Dawley malo rats weighting 250 ?300 g were randomly diviged into three groups （ n = 6 each） : sham operation group （ group S） , ischemia/reperfusion group （ group I/R） and ozone oxidative preconditioning group （ group O3 + I/R）。 In Group O3+I/R, rats received five-day preconditioning treatments by  intraperitoneal injection of ozone and oxygen mixed gases （ ozone concentration 50 mg/L, 1 mg ? kg -1 ? d -1 ） , and then experienced the procedure of he-patic ischemia/reperfusion injury. Model of  hepatic ischemia/reperfusion injure was established by clamping the branches of  hepatic artery and portal vein in the median and left lateral hepatic lobes for 45 min, followed by 3-h reperfusion. After reperfusion, blood samples were taken from the aorta abdominalis for detecting ser- um aminotransferases （ ALT & AST）。 Thess rats were executed and the hepatic tissue samples were collec-ted for measuring hepatic malondialdehyde（ MDA）and superoxide dismutase （ SOD） level。 Results Compared with group S, concentrations of serum ALT, AST and hepatic MDA were increased in group I/R and O3+I/R； concentrations of  hepatic SOD were decreased （ P<0. 05） in group I/R, but concentration of  hepatic SOD was in creasedin groupO3+I/R Compared with group IR, concentrations of serum  ALT,AST and hepatic MDA were decreased, while concentration of hepatic SOD was increased in group O3+I/R （P < 0. 05 ） . Conclusion Ozone oxidative preconditioning could inhibit the lipid peroxidation  to protect the rats against hepatic ischemia/reperfusion injury.

　　〖Key words〗Ozone oxidative preconditioning；Ischemia/reperfusion injury；Liver

　　Frnd program: Natural Sciencc Foundation of  Fujian  Province （2016J01543）

　　DOI：lO.376O/cma. j.issn. 1007-8118.2017.03.014

　　作者單位：350028福州，福建省立醫院南院麻醉科（李糧輝）；福建醫科大學附屬協和醫院麻醉科（陳文華），肝膽外科（陳燕凌）；福建中醫藥大學附屬人民醫院麻醉科（李麗珍）；福建省腫瘤醫院麻醉科（王蘭蘭）

　　通信作者：陳文華，電子信箱：whc6202@163.com

　　未完待續......